DCAMKL1, active Human Recombinant Kinase Protein
N-terminal 6His-tagged recombinant, human DCAMKL1 full length.
No items found.
Biological information
Background
DCAMKL1 is a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity.
GenBank NM_004734.2
Target class
Kinase
Family
CAMK
Accession number
NM_004734.2
Target Name
DCAMKL1, active Human Recombinant Kinase Protein
Target Alias
Origin
Human
Theori. MW
85 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 72% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
DCAMKL1 is a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity.
GenBank NM_004734.2
Compound name
Kinase
Catalog number
14-986
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Kinase Activity Assay Biological information
Background
DCAMKL1 is a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity.
GenBank NM_004734.2
Target class
Kinase
Family
CAMK
Subfamily
Protein Name
DCAMKL1
Protein Alias
Accession Number
NM_004734.2
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
DCAMKL1 is a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity.
GenBank NM_004734.2
Target class
Kinase
Family
CAMK
Accession Number
NM_004734.2
Target Name
DCAMKL1
Target Alias
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 72% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Biological Information
Background
DCAMKL1 is a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity.
GenBank NM_004734.2
Target class
Kinase
Family
CAMK
Accession Number
NM_004734.2
Target Name
DCAMKL1
Target Alias
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 72% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only