GRK3, active Human Recombinant Kinase Protein
10 µg of active GRK3. C-terminal 6His-tagged, recombinant, full length, human GRK3.
No items found.
Biological information
Background
GRK3, G protein-coupled receptor kinase 3, is a beta-adrenergic receptor kinase that specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Accession number
NM_005160.4
Target Name
GRK3, active Human Recombinant Kinase Protein
Target Alias
G Protein-Coupled Receptor Kinase 3; BARK2; ADRBK2; Adrenergic, Beta, Receptor Kinase 2; Beta-ARK-2
Origin
Human
Theori. MW
82 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
94% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
3.31 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
GRK3, G protein-coupled receptor kinase 3, is a beta-adrenergic receptor kinase that specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]
Compound name
Kinase
Catalog number
15-028
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Kinase Activity Assay Biological information
Background
GRK3, G protein-coupled receptor kinase 3, is a beta-adrenergic receptor kinase that specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Subfamily
Protein Name
GRK3
Protein Alias
G Protein-Coupled Receptor Kinase 3; BARK2; ADRBK2; Adrenergic, Beta, Receptor Kinase 2; Beta-ARK-2
Accession Number
NM_005160.4
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C.
Usage disclaimer
Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
GRK3, G protein-coupled receptor kinase 3, is a beta-adrenergic receptor kinase that specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Accession Number
NM_005160.4
Target Name
GRK3
Target Alias
G Protein-Coupled Receptor Kinase 3; BARK2; ADRBK2; Adrenergic, Beta, Receptor Kinase 2; Beta-ARK-2
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
94% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Biological Information
Background
GRK3, G protein-coupled receptor kinase 3, is a beta-adrenergic receptor kinase that specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Accession Number
NM_005160.4
Target Name
GRK3
Target Alias
G Protein-Coupled Receptor Kinase 3; BARK2; ADRBK2; Adrenergic, Beta, Receptor Kinase 2; Beta-ARK-2
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
94% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only