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LIMK2, active Human Recombinant Kinase Protein

10 µg of active LIMK2. N-terminal 6His-tagged, recombinant, human LIMK2 amino acids 2-end.

Biological information

Background
LIMK2, LIM domain kinase 2, is a serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. This kinase has also been implicated in brain development. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
TKL
Accession number
NM_005569.4
Target Name
LIMK2, active Human Recombinant Kinase Protein
Target Alias
LIM Domain Kinase 2; LIMK-2
Origin
Human
Theori. MW
76 kDa
Affinity tag
6His

Product specifications

Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
82% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
0.65 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only

Chemical data

Background
LIMK2, LIM domain kinase 2, is a serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. This kinase has also been implicated in brain development. [provided by RefSeq, Jul 2008]
Compound name
Kinase
Catalog number
16-032
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure

Kinase Activity Assay Biological information

Background
LIMK2, LIM domain kinase 2, is a serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. This kinase has also been implicated in brain development. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
TKL
Subfamily
Protein Name
LIMK2
Protein Alias
LIM Domain Kinase 2; LIMK-2
Accession Number
NM_005569.4
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human

Kinase Activity Assay Usage

Product Type
Application
Storage Conditions
1 year at -70°C.
Usage disclaimer

Kinase Activity Assay Information

Assay Type
Assay Measures

Biological Information

Background
LIMK2, LIM domain kinase 2, is a serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. This kinase has also been implicated in brain development. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
TKL
Accession Number
NM_005569.4
Target Name
LIMK2
Target Alias
LIM Domain Kinase 2; LIMK-2
Origin
Human
Theoretical MW
Affinity Tag
6His

Product Specifications

Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
82% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only

Biological Information

Background
LIMK2, LIM domain kinase 2, is a serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. This kinase has also been implicated in brain development. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
TKL
Accession Number
NM_005569.4
Target Name
LIMK2
Target Alias
LIM Domain Kinase 2; LIMK-2
Origin
Human
Theoretical MW
Affinity Tag
6His

Product Specifications

Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
82% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Datasheets
User Manual
File N°2
€ 389,00 EUR
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