MEKK3, active Human Recombinant Kinase Protein
10 µg of active MEKK3. N-terminal GST-tagged, recombinant, human MEKK3 full length.
No items found.
Biological information
Background
MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Ser/Thr Kinase
Accession number
NM_002401.3
Target Name
MEKK3, active Human Recombinant Kinase Protein
Target Alias
Mitogen-Activated Protein Kinase Kinase Kinase 3MAPK/ERK Kinase Kinase 3MAP/ERK Kinase Kinase 3MEK Kinase 3EC 2.7.11.25
Origin
Human
Theori. MW
97.7 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 52% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified using glutathione agarose.
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. [provided by RefSeq, Jul 2008]
Compound name
Kinase
Catalog number
16-015
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Kinase Activity Assay Biological information
Background
MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Ser/Thr Kinase
Subfamily
Protein Name
MEKK3
Protein Alias
Mitogen-Activated Protein Kinase Kinase Kinase 3MAPK/ERK Kinase Kinase 3MAP/ERK Kinase Kinase 3MEK Kinase 3EC 2.7.11.25
Accession Number
NM_002401.3
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C.
Usage disclaimer
Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Ser/Thr Kinase
Accession Number
NM_002401.3
Target Name
MEKK3
Target Alias
Mitogen-Activated Protein Kinase Kinase Kinase 3MAPK/ERK Kinase Kinase 3MAP/ERK Kinase Kinase 3MEK Kinase 3EC 2.7.11.25
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 52% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified using glutathione agarose.
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Biological Information
Background
MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Ser/Thr Kinase
Accession Number
NM_002401.3
Target Name
MEKK3
Target Alias
Mitogen-Activated Protein Kinase Kinase Kinase 3MAPK/ERK Kinase Kinase 3MAP/ERK Kinase Kinase 3MEK Kinase 3EC 2.7.11.25
Origin
Human
Theoretical MW
Affinity Tag
GST
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 52% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified using glutathione agarose.
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only